LOCUS       sequence-53719-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt3-V5
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac ccttcagaat
      121 gcacttgcag gtgtgcacgt cgtagatgcg aatacactcc tggcagctga cgtagcagca
      181 ccagtggaag atgcagtggc atttttcctt ccgcttctcc gtcctcgtgt tgtggccccg
      241 gccacagcag agcagatcgc agccatcgat gccgtgggag gtgacattgc aagtccggtc
      301 ccttgtgcca aaggaacccg tctctgggtt gggctcacaa aagttggggg agttctcgta
      361 gtagaccagg tccctctccg tgggtggctt gaagagcgag tacttggccc ggagggtctc
      421 cacccagcct cgggactcac ggtgcttctc tactaccatc tccgaggcgc tgtcatactt
      481 gtccttgagg aagtcaccga tggcacggaa gtcaggctgc gcccaccagc aggtcttcac
      541 ctcacagctg cccgacagcc cgtggcactt gcatttgagg tgcatgtggt ccaggatagt
      601 cgtgcggccc gcctcgttgt tgtgcttgtt catggccgag cgcgcgtccg gcctgttctc
      661 gcgcgcatcc gcgaactccc tggacactaa cacgccgaag tcagcgtcct cgctgcagcc
      721 gccccacttc cagccttcgc caggcggccc cttatgatgc gag
//
