LOCUS       sequence-53722-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRS40B
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    22..129
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     38..54
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     39..55
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(89..105)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(89..105)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(142..162)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(142..160)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(181..197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(181..197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(194..216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    205..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(229..235,236..253,254..259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    274..295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(412..429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(663..682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
       61 cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg
      121 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
      181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      721 gccgcttacc ggatacctgt ccgcctttct cccttcgg
//
