LOCUS       sequence-53727-e        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Conformation-Selective ATP-Competitive Inhibitors Control Regulatory
            Interactions and Noncatalytic Functions of Mitogen-Activated Protein
            Kinases.
  JOURNAL   Chem Biol. 2014 May 22;21(5):628-35. doi: 
            10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.
  PUBMED    24704509
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.02.016"; journalName: "Chem Biol"; date: 
            "2014-05-22- 22"; volume: "21"; issue: "5"; pages: "628-35"
COMMENT     Sequence Label: pcDNA3-eGFP-LIC
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(74..92)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(75..92)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(112..129)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    118..342
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      388..816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(475..494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     685..706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(825..845)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctcggcatgg angagctgta caagcagctt cagggatcgt acttccaatc caatattgga
       61 agtggataac tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
      121 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      181 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      241 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      301 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
      361 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
      421 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      481 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      541 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      601 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      661 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      721 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      781 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
      841 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      901 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcnncagaag tatgcaaagc
      961 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctcgcccatc ccgcccctaa
     1021 ctcncccann nncccatnnt cgcccatggc tgantnnttt ttttantnat gcagangccg
     1081 agcnncctcn ncntnnganc tantncagaa nnnnnnangn nngctttttt tg
//
