LOCUS       sequence-53764-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Muona M, Aranko AS, Iwai H
  TITLE     Segmental isotopic labelling of a multidomain protein by protein 
            ligation by protein trans-splicing.
  JOURNAL   Chembiochem. 2008 Dec 15. 9(18):2958-61.
  PUBMED    19031436
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2008 Dec 15. 9(18):2958-61."
COMMENT     Sequence Label: pHYRS52
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc
       61 atggtctggt tccgcgtgga tcccttgttc ctgaattaaa tgaaaaagac gatgaccaag
      121 tacaaaaagc tttggcatct agagaaaata ctcagttaat gaatagagat aatatagaga
      181 taacagtacg tgattttaag accttggcac cacgaagatg gctaaatgac actatcattg
      241 agttttttat gaaatacatt gaaaaatcta cccctaatac agtggcgttt aattcgtttt
      301 tctataccaa tttatcagaa aggggttatc aaggcgtccg gaggtggatg aagagaaaga
      361 agacacaaat tgataaactt gataaaatct ttacaccaat aaatttgaac caatcccact
      421 gggcgttggg cataattgat ttaaaaaaga aaactatagg ttacgtagat tcattatcga
      481 atggtccaaa tgctatgagt ttcgctatac tgactgactt gcaaaaatat gttatggagg
      541 aaagtaagca tacaatagga gaagactttg atttgattca tttagattgt ccgcagcaac
      601 caaatggcta cgactgtgga atatatgttt gtatgaatac tctctatgga agtgcagatg
      661 cgccattgga ttttgattat aaagatgcga ttaggatgag aagatttatt gcccatttga
      721 ttttaaccga cgctttaaaa taagaattcg aagcttgatc cggctgctaa caaagcccga
      781 aaggaagctg agttggctgc tgccaccgct gagcaataac
//
