LOCUS       sequence-53774-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52 LRRK2_BC_1520_2148_I2149ENLYFQGWSHPQFEK
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccgga
      121 cagctgatcc ccgactgcta cgtggaactt gagaagatca tcctgagcga gcgcaagaat
      181 gtgcctatcg agtttccagt gatcgatcgc aaacgtctcc tccagctcgt gcgtgagaat
      241 cagctgcagc tcgacgagaa cgagctgcct catgctgtgc acttccttaa cgagtcaggt
      301 gtcctcctgc acttccagga cccggctctc cagctgagtg acctctattt cgtggagcca
      361 aagtggctct gtaaaattat ggctcaaatc ctcactgtca aggtagaggg ctgtccaaag
      421 cacccaaagg gcatcatctc gcgtcgcgac gtggaaaagt ttctctctaa gaagcgcaag
      481 tttcccaaga actacatgag ccagtacttt aaactgttgg agaagttcca gatcgctctg
      541 cctatcggcg aagagtacct gttggttcct tcctccctgt ccgatcaccg ccccgtgatt
      601 gagctgcctc actgcgagaa cagcgagatt atcattcgcc tatacgagat gccgtacttt
      661 ccgatgggct tctggtcaag gctcatcaat cgtctgcttg aaatctcccc atatatgctg
      721 agcggtcgtg aacgcgctct gaggccaaac cggatgtact ggcgccaggg gatttacttg
      781 aactggtccc ctgaggctta ttgtctggtg ggctcggaag tcctgg
//
