LOCUS       sequence-53775-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52 LRRK2_BC_1520_2148_I2149ENLYFQGWSHPQFEK
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..65)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(66..86)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggtggtggtg gtggtgctcg agtgcggccg caagcttatc acttctcgaa ctgggggtgg
       61 gaccagccct ggaagtacag gttctccttc gggagtaaga tacgcctagt cagacacact
      121 aactctgcag agtttagaat gtcgaacacc tgggcgcttg tggggcgttc ctgagggttc
      181 tccttcagac actgcttaat gagcttctcc accataggcc agggtgcaca accgtactcc
      241 ttcacggggt caggcagctt gccctggatc tccagctcgt cgaactcgtt gggaaatttc
      301 aggccctcca caatccggcc tccggtagtg agaatgtcat acaggagcag gccaaacgag
      361 tacacgtcgg cctgctggtt gtagatcacg ttcccgcgtg cgacttcggg agcgcggaac
      421 ccaggtgtac cctcgctagt cttgataccc atacgacagc agtactgggc tatgccgtaa
      481 tcggcaattt tggcaatgat agctgcgttt gggtagagcg tgaacagaag aacattatga
      541 ggcttcagat cgcgataaat gatcatggca ctgtgcaagt agcgcagtcc gtcggccaca
      601 tgcagggcga tacggtgctg gagcgtacgg gtcaggctag ccttgtcctg ctgcagcagg
      661 cggtccaatg aacccttact ggccagttcc atcactagca tgcgaggcct aatgccggcg
      721 gccagcaggg
//
