LOCUS       sequence-53777-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label:  pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEK
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(67..87)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tggtggtggt ggtggtgctc gagtgcggcc gcaagcttat cacttctcga actgggggtg
       61 ggaccagccc tggaagtaca ggttctcctc aacaggcaga tggacaaggg cgaggcacag
      121 aatgcgtgaa tcagcgacct cctcgctagt atacccctcg gtgttgaggt cgaggaaact
      181 cagctggcca cgatcggtat gcccacatcc taaccagatg ctagcgttac gcgagttgtg
      241 gtgcgttgcc accatgcact caacgatcac gttcttcggg agtaagatac gcctagtcag
      301 acacactaac tctgcagagt ttagaatgtc gaacacctgg gcgcttgtgg ggcgttcctg
      361 agggttctcc ttcagacact gcttaatgag cttctccacc ataggccagg gtgcacaacc
      421 gtactccttc acggggtcag gcagcttgcc ctggatctcc agctcgtcga actcgttggg
      481 aaatttcagg ccctccacaa tccggcctcc ggtagtgaga atgtcataca ggagcaggcc
      541 aaacgagtac acgtcggcct gctggttgta gatcacgttc ccgcgtgcga cttcgggagc
      601 gcggaaccca ggtgtaccct cgctagtctt gatacccata cgacagcagt actgggctat
      661 gccgtaatcg gcaattttgg caatgatagc tgcgtttggg tagagcgtga acagaagaac
      721 attatgaggc ttcagatcgc gataaatgat catggcactg tgcaagtagc gcagtccgtc
      781 ggccacatgc agggcgatac ggtgctggag cgtacgggtc aggctagcct tgtcctgctg
      841 ca
//
