LOCUS       sequence-53787-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB403 Urchin_LRRK2_1_335
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..97
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..748
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     706..728
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             755..772
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatctatgc atcatcatca ccatcactcc cctatactag gttattggaa
      121 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
      181 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
      241 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
      301 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
      361 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
      421 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
      481 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
      541 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
      601 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
      661 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
      721 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtggat ccggccgtaa
      781 agtgccgcta cagatcctgg ccccggatgt caccttagcg gacctggagg acaagttttt
      841 actggaccct cgaaaattaa acttcaccca gcgcgccgaa aatctcctgg gcgatggcag
      901 ctttgggtcc gtttacaagg
//
