LOCUS       sequence-53796-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2350
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..100
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             101..118
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctcgcaaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
       61 aaatatagga tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatccga
      121 cgagctgcac ctaaacttcg acttcaagca cattggctgt aaagcaaagg atattattcg
      181 tttcctgcag cagcgtctta agaaggccgt gccctacaat cgcatgaagc tgatgatcgt
      241 cgggaacacc ggttctggaa agacaaccct cttgcagcag cttatgaaga ctaagaagtc
      301 cgacttgggg atgcagagcg ccactgtggg gatcgacgtg aaagactggc caatccaaat
      361 ccgtgacaag cgcaagcgtg acttagtact gaacgtgtgg gactttgccg gcagggaaga
      421 gttctactct acgcatcctc acttcatgac tcagcgtgcc ctctatctgg cagtttacga
      481 cctctcaaag ggccaggctg aggtggacgc catgaaaccc tggctgttta acatcaaggc
      541 tcgtgcttcc agctcccctg tgatcttagt cgggacacac ttggatgttt ctgatgagaa
      601 gcagcgtaag gcatgtatga gtaagatcac caaagagctg ttgaacaaac gcggctttcc
      661 cgccatccgt gattaccact tcgtcaacgc taccgaggaa agcgacgcac tggccaagtt
      721 acgtaagacc attatcaacg aaagcctgaa cttcaaaatt cgcgaccagc tggtggtcgg
      781 acagctgatc cccgactgct
//
