LOCUS       sequence-53797-e         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Zheng L, Njauw CN, Martins-Green M
  TITLE     A one-plasmid conditional color-switching transgenic system for 
            multimodal bioimaging.
  JOURNAL   Transgenic Res. 2008 Aug;17(4):741-7. Epub 2008 Jan 9.
  PUBMED    18183494
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res."; date: "2008-08"; volume: "17(4)"; pages: "741-7. Epub 2008 
            Jan 9"
COMMENT     Sequence Label: pRLG3A
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(205..238)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(388..406)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattcctcg aggaattcac tcctcaggtg caggctgcct atcagaaggt
      121 ggtggctggt gtggcgcgtg ctagtaacgg ccgccagtgt gctggaattc tgcagatagc
      181 ttgggctgca ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat
      241 taagggttcc ggatccgatg gcctcctccg aggacgtcat caaggagttc atgcgcttca
      301 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      421 ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc
      481 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      541 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaagactcc tccctgcagg
      601 acggcgagtt catctacaag gtgaagctgc gcggcacc
//
