LOCUS       sequence-53812-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB44 / Zebrafish_LRRK2_1_338
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..130
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             131..148
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
       61 cctataaata taggatccag gcctgaattc gagctccaag cttatgcacc accaccatca
      121 ccatcatcat ttagttcctc gcggctcagg atgcacgtta cctatatcgc agatttcacc
      181 ggacctggtg ctttctgacc agccttcctc gacaatcctg gatccagagc agttagaaat
      241 ggaactaacc gctgagtata tgctcggcga tggaggcttc ggcagtgttt ataaagccgt
      301 gtacaaaaac gaggaggtgg cggtcaagat ctttaacaaa cacgcatcgg ctttgtatgt
      361 gcaccgctta gtccgccagg agctagccgt attagggcgt ctctgtcatc cctcgctggt
      421 cggcctgtta gcggccggat gcaatcccca tatcctcgta atggagctgg caccgtacgg
      481 cagtctggac tcgctcttcg aacgagaaaa cggttccttg tcaagaaaat tgcaacaccg
      541 tattgcgttg cacgtcgccg acgggcttaa atacttgcat agttcaatga tcatctaccg
      601 ggacctgaaa ccgcataacg tactgctttt taatcttaag accgatgctg agattgttgc
      661 aaaaattacg gattacggca tcgcacagta ttgttgtagt atgggcgtcc ggtcatctga
      721 aggaaccccg ggtttccgtg cgcctgaagt ggcccgcggg aacgtgattt ataatgtgca
      781 ggcagatgtt tatagcttcg gtctgctgct gtatgacctg ctgacctatg gtgaacgcat
      841 ttccgatggc atgaaatttc cgtct
//
