LOCUS       sequence-53814-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB44 / Mouse_LRRK2_1_342
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..115
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             116..133
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatccaggc ctgaattcat gcaccaccac catcaccatc atcatttagt
      121 tcctcgcggc tcaggcctga caatccctat ctctcagata gcaccggatc taatcttggc
      181 cgatttgccg cgaaacatca tgcttaataa cgacgaactc gagttcgagg aagcgccgga
      241 atttttgttg ggagatggct ccttcggcag tgtctaccgt gcggcttatg agggtgagga
      301 agttgctgtt aaaatcttta ataaacacac ctcgcttaga ctattgcgac aagagctggt
      361 ggttctttgc catctgcatc atccaagcct cataagtttg ttagcagccg gtatccggcc
      421 tcgtatgtta gtgatggagt tagcctctaa agggtccctc gaccgccttc tccagcagga
      481 caaagcaagt cttactcgta ccctccagca tcgtatcgct ctgcatgtgg ctgacggctt
      541 acgctacctg catagcgcca tgattattta tcgcgatctg aaaccacata acgtcttact
      601 gtttactctg taccccaatg ccgcaatcat tgcgaaaatc gccgattatg gcattgcaca
      661 atattgctgc cgtatgggca ttaagaccag cgagggtaca ccaggttttc gcgcgcccga
      721 agtggcacgt gggaacgtta tctataatca gcaggcggat gtatactcgt ttgggttact
      781 gctgcacgac atctggacaa ccggtagccg cattatggaa gggctgcgtt tcccgaacga
      841 atttgatgaa ct
//
