LOCUS       sequence-53817-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y,
            Yang W, Gruber PJ, Epstein JA, Morrisey EE
  TITLE     Highly efficient miRNA-mediated reprogramming of mouse and human 
            somatic cells to pluripotency.
  JOURNAL   Cell Stem Cell. 2011 Apr 8. 8(4):376-88.
  PUBMED    21474102
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2011 Apr 8. 8(4):376-88."
COMMENT     Sequence Label: pLOVE-miR302/367
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(809..833)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    874..892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    895..913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(915..959)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 gactcgagcg gccgccacct gtgcctggat atcaaccact ttgtacaaga aagctgggtt
       61 ctagatatct cgagtgcggc cgcgaattcg tcgacaacac aaggagagac ataagatggg
      121 cgaggaggtt ataccattga gagtgctgtc caagtaagtg gccactgaca caggtccatc
      181 accattgcta aagtgcaatt ccaatttcta ttcaacagag ttgcatgtta gcaacagtga
      241 cggccttggt ctatctatat gcagcacaat gaagattaag taggagccac cacactcaaa
      301 catggaagca cttattttta aatgcacagc aagtgcctcc atgttaaagt aaaggaggcc
      361 ccttaagcag acttgagtgg tctttacaaa ggcatgaata taaaggaaaa cataccatca
      421 ccaaaacatg gaagcactta ctttcttagg tctaaagcaa gtacaaccac gtttaagtgg
      481 tgggaagccc agtcaataac tcccttaacc tacagcatct tagagaaaat gttgaacagt
      541 ttaagacacc cccactgaaa catggaagca cttgcttttg tttaacacag caggtaaccc
      601 catgttaaag cagaggggac tcccatcaga ctctgaatta aaaggctagc tttcttattc
      661 cagagtttct tgaattcact tctactaaaa catggaagca cttactcttc gatgagacag
      721 aaagcattcc catgttaaag ttgaagggaa cccacccaca aggcaactag ttaaaacaga
      781 agagcgtcga ctgaattggt tcctttaaag cctgcttttt tgtacaaact tgttgatatc
      841 tgcagaattc caccacactg gactagtcga cgatctctat cactgatagg gagatctcta
      901 tcactgatag ggagagctct gcttatatag acctcccacc gtacacgcct accgcccat
//
