LOCUS       sequence-53819-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB44 / Lancelet_LRRK2_1_319
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..115
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             116..133
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatccaggc ctgaattcat gcaccaccac catcaccatc atcatttagt
      121 tcctcgcggc tcaggccacg atatgccagt acccttacac aaactagcac cggatgtcat
      181 gctcagtgat ctggattcgc gctaccagct ccaagaagat aaatttaaac ttgagcaaat
      241 tcccgagaac gagttaggcg atgggagttt tgggaacgtg tatcgcgcta gttataagaa
      301 tatggacgtt gcagtgaagg tcttttcgaa ggtaggtgag gatcatccac accgcatgct
      361 tcgtcaagag gtaacgatcc tgactcgtct tcagcatata tcggtcatct cattactagc
      421 cgtgggcatt cgcccgcggt tgttagtgtt agaacttgca ccgatgggtt ccttgacctc
      481 ccacttgtgc aagaaaatga gccgcggcct gcagcagcgc attgccatgc aaatagccga
      541 aggattgatg ttcttgcata caaacatgat catctaccga gacctcaaac cagacaacat
      601 tctgattttc agcatcgctc agaactctcc cgtgaacgcg aaaatctccg actatggtat
      661 ttctaaattt gctactctcc tgggtctgac agcgccagaa ggtacgcctg gctaccgtgc
      721 gccggaagtg gcccgtggtg atacggcgta taataataaa gtcgacctgt tctcatttgg
      781 tatcctgctg tttgaactgg tgaccgggcg taaacctttc gaagatctgc atttccgtgg
      841 cgaactggat gaagccatta tcaaaggacg tcctctggat c
//
