LOCUS       sequence-53822-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52 / Urchin_LRRK2_1_335
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccggc
      121 cgtaaagtgc cgctacagat cctggccccg gatgtcacct tagcggacct ggaggacaag
      181 tttttactgg accctcgaaa attaaacttc acccagcgcg ccgaaaatct cctgggcgat
      241 ggcagctttg ggtccgttta caaggggacg taccgggata aacctgtggc cgtcaaagtg
      301 tttagtaaag ggggtgaatc tcacccgcac cgacttttgc ggcaggaagt tactgtgctg
      361 cgccacctgc aacatccgag tttagtatca atggaggggg tgggtctgaa cccgcgcatt
      421 ctggtcatgg agcttgcgcc cctgggatct ttgaacacgc tgctggcgag tggaagaatc
      481 aagaaccgta acctgcaaca ccgaatagcc atgcaggtgg cggagggact ggccttcctg
      541 cataaaaacc gtatcgtata ccgtgatcta aaaccagaca acgtactgat tttcagtctt
      601 gccttaggca cactgcagaa tgcgaaaatt tcggattacg gtattagccg tttcgctacc
      661 ccctacggct taaaatcctc tgagggcaca ccgggctatc gcgctccaga agtaatccgg
      721 ggcaccagca attataatac agaagtggac atctattcat ttggtatctt gctctatgaa
      781 ctggtctcag agggtcaccg cccattcgaa gagatggaat tccgtaccga aatcgatgaa
      841 gctgttatta aaggccagca tctgccgccg atct
//
