LOCUS       sequence-53824-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52 / Anemone_LRRK2_1_334
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccgtc
      121 attatgcctc tccaacatgt agcacctgat gtggtcttag cagatctaga tcagcagtgg
      181 atgttggact cggctgagtt atgtctggaa gagaagaagg aaaatgtatt aggtgacggt
      241 ggttatggaa cggtttacaa agcgaaatac tatggacagt ttgtcgcggt aaaagtattt
      301 cgcaaggcga cagacgtttg cccccatcgg cttcttagac aagaagttcc gtttctatcc
      361 cggctgaggc acccaagcat cataaatatg atcggagttg ccttgaaacc gcgagcactt
      421 gtgcttgagc tcgctccatt gggttccttg agtagcctgc tcagtaaggg cgagggcctg
      481 tcacgtggcc tgcaacaccg tattgcatta caggtggccg aaggcctggc ctatttgcac
      541 aaacacatga tcgtctaccg tgacctcaaa cctcataaca tcctgatttt tagtctgtct
      601 ttagggattt taattaatgc caaaatctcg gattacggca ttgctcgcta tgcgacgctg
      661 tacgggctga ccgcacccga agggacccca ggctatcgcg cgccggaagt tgcccgcggg
      721 gacgtcgcct ataacaaaga agcggatatg tattcattcg gtatgttcct gtacgaactg
      781 gtgaccggcg gtaaacgtcc gtttgaagac tg
//
