LOCUS       sequence-53826-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52 / Human_LRRK1_1_360
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccgga
      121 ctgcctgtcc cgctgcagga gctggttcct gaactgttca tgaccgattt ccccgctcgc
      181 ttgtttctgg agaatagtaa attggaacac tcagaggatg agggtagcgt gctggggcaa
      241 ggtgggtcag ggaccgtgat ctatcgtgcg cgttaccaag gacagcccgt cgctgtgaag
      301 cgctttcata tcaagaagtt caaaaatttc gcaaatgtgc ctgctgatac gatgttacgt
      361 catcttcggg ctacagatgc catgaaaaat ttctctgaat ttcggcaaga agcctctatg
      421 ttacatgccc tgcaacaccc gtgtatcgtg gccttgatcg gcattagcat tcatccgctc
      481 tgcttcgcct tagaactggc accgctgtct tccctgaata ccgtattaag tgaaaacgcc
      541 cgtgacagct cgttcatccc actgggtcat atgcttaccc agaagatcgc atatcaaata
      601 gcgtcgggcc tggcatatct gcacaagaaa aacattattt tttgtgattt gaaatcggac
      661 aacattttag tgtggtcgct agacgtgaaa gaacacatca acattaaact cagcgactac
      721 ggtatttcgc gccagagctt tcacgaagga gcgttgggcg tagaaggtac tccaggttat
      781 caagcaccgg aaaattcgcc
//
