LOCUS       sequence-53838-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger 
            P, Rothballer A, Ascano M Jr, Jungkamp AC, Munschauer M, Ulrich A, 
            Wardle GS, Dewell S, Zavolan M, Tuschl T
  TITLE     Transcriptome-wide identification of RNA-binding protein and 
            microRNA target sites by PAR-CLIP.
  JOURNAL   Cell. 2010 Apr 2;141(1):129-41.
  PUBMED    20371350
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2010-04-2"; volume: "141(1)"; pages: "129-41"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST PUM2
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..164
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             165..191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    207..231
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgag ctcggtcgat ccatcgatag tgtggtggaa
      121 ttctgatcgc cgccgccatg gactacaagg acgacgatga caagtaccct tatgacgtgc
      181 ccgattacgc tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg
      241 gaaccatgaa tcatgatttt caagctcttg cattagaatc tcggggaatg ggagagcttt
      301 tgcctaccaa aaagttttgg gaacctgatg attcaacaaa agatggacaa aaaggcatat
      361 ttcttgggga tgatgaatgg agagagactg catggggagc ttctcaccat tcaatgtccc
      421 agcctattat ggtacagaga agatctggac agggttttca tggaaacagt gaagtaaatg
      481 caatactgtc tccgcgatca gaaagtggag gccttggtgt gagcatggta gaatatgtat
      541 taagttcttc tcctgctgat aaattggatt ctcgatttag gaagggaaat tttggcacta
      601 gagatgctga aacagatgga cctgagaaag gagatcaaaa aggcaaggct tctccatttg
      661 aggaggacca aaacagagac cttaaacaag gagatgatga tgattctaaa ataaatggca
      721 gaggtttgcc aaatggaatg gatgccgatt gcaaagattt taatcgtact cctggaagtc
      781 gtcaagcctc tccaactgaa gtagttgagc gcttgg
//
