LOCUS       sequence-53841-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Nemoto S, Fergusson MM, Finkel T
  TITLE     Nutrient availability regulates SIRT1 through a forkhead-dependent 
            pathway.
  JOURNAL   Science. 2004 Dec 17. 306(5704):2105-8.
  PUBMED    15604409
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Dec 17. 306(5704):2105-8."
COMMENT     Sequence Label: pTA-Luc SIRT1 promoter (-202)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        248..279
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(407..425)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gatagcaggc cgcgggcggc gcccggccac gtgacccggc gtgttgtggt
       61 ccggcccgcg tgggtggcgg gagaggcgcg ggggcgggga ggggcgggtc acgtgacggg
      121 gtttaaatct cccgcagccg agccgcgggg gcgccagtgc cgcgcgtcga gcggagcaga
      181 ggaggcgagg gcggagggcc agagaggcag ttggaagaag ggcgaattct gcagatgatc
      241 tagactctag agggtatata atggaagctc gaattccagc ttggcattcc ggtactgttg
      301 gtaaaaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      361 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      421 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      481 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      541 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      601 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      661 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      721 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
      781 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      841 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
      901 tagggaca
//
