LOCUS       sequence-53844-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kim KH, An DR, Song J, Yoon JY, Kim HS, Yoon HJ, Im HN, Kim J, Kim 
            do J, Lee SJ, Kim KH, Lee HM, Kim HJ, Jo EK, Lee JY, Suh SW
  TITLE     Mycobacterium tuberculosis Eis protein initiates suppression of host
            immune responses by acetylation of DUSP16/MKP-7.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr
            30.
  PUBMED    22547814
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-05-15"; volume: "109(20)"; pages: 
            "7729-34. Epub 2012 Apr 30"
COMMENT     Sequence Label: pET21a-MKP7_K52R_153
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             493..515
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    530..554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(554..573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(555..573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(657..676)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagag gtaagcaagg ctgagaaatg
       61 caggtaggga ctagagtgga ttttccttca cagagaccag ggaaacaacg agagaactca
      121 gcaaacccac ctgcaagcag gtgaacagag ttgaagctct tctccagttt acccagaagt
      181 acagtgagaa aacagtctga agagagagag gcaacatctt gggagctttg atcgtaaact
      241 acaaccttct gactgcaatc aatgtcaacc ttatgtttcg ctgaatgctg gatgagctct
      301 gtaattaaca ctttgtcctg ttgcaacctt cgcttcataa ggcgggagca gttgatatta
      361 atggcttcca aaatgtggga tgtattgtat tccacaaatg gccggctatc aattagcagc
      421 actttttccg ttccactttc cagcagagcc accaacctct cagtaacaat ttgagttcca
      481 atcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      541 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      601 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      661 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      721 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      781 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      841 cctaatgcag gagtcgcata agggagagcg tcgag
//
