LOCUS       sequence-53845-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Kim KH, An DR, Song J, Yoon JY, Kim HS, Yoon HJ, Im HN, Kim J, Kim 
            do J, Lee SJ, Kim KH, Lee HM, Kim HJ, Jo EK, Lee JY, Suh SW
  TITLE     Mycobacterium tuberculosis Eis protein initiates suppression of host
            immune responses by acetylation of DUSP16/MKP-7.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr
            30.
  PUBMED    22547814
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-05-15"; volume: "109(20)"; pages: 
            "7729-34. Epub 2012 Apr 30"
COMMENT     Sequence Label: pET21a-MKP7_K55R_153
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             502..524
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    539..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(563..582)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(564..582)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(666..685)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggcttgttg cagccggatc tcagtggtgg tggtggtggt gctcgagagg taagcaaggc
       61 tgagaaatgc aggtagggac tagagtggat tttccttcac agaggccagg gaaacaacga
      121 gagaactcag caaacccacc tgcaagcagg tgaacagagt tgaagctctt ctccagttta
      181 cccagaagta cagtgagaaa acagtctgaa gagagagagg caacatcttg ggagctttga
      241 tcgtaaacta caaccttctg actgcaatca atgtcaacct tatgtttcgc tgaatgctgg
      301 atgagctctg taattaacac tttgtcctgt tgcaaccttc ggcgcataag cttggagcag
      361 ttgatattaa tggcttccaa aatgtgggat gtattgtatt ccacaaatgg ccggctatca
      421 attagcagca ctttttccgt tccactttcc agcagagcca ccaacctctc agtaacaatt
      481 tgagttccaa tcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      541 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      601 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      661 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      721 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      781 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      841 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
      901 aatggcgcaa aacctttcgc
//
