LOCUS       sequence-53855-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Wu B, Chao JA, Singer RH
  TITLE     Fluorescence fluctuation spectroscopy enables quantitative imaging 
            of single mRNAs in living cells.
  JOURNAL   Biophys J. 2012 Jun 20;102(12):2936-44. Epub 2012 Jun 19.
  PUBMED    22735544
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2012-06-20"; volume: "102(12)"; pages: "2936-44. Epub 
            2012 Jun 19"
COMMENT     Sequence Label: phage-ubc-nls-ha-tdPCP-gfp
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             399..419
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             423..449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             423..449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             450..461
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
       61 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
      121 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
      181 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
      241 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
      301 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
      361 tggatcgctg tgatcgtcac ttggcggccg ccatgggccc aaaaaagaaa agaaaagttg
      421 gctaccccta cgacgtgccc gactacgcca tcgaaggccg ccatatgcta gcctccaaaa
      481 ccatcgttct ttcggtcggc gaggctactc gcactctgac tgagatccag tccaccgcag
      541 accgtcagat cttcgaagag aaggtcgggc ctctggtggg tcggctgcgc ctcacggctt
      601 cgctccgtca aaacggagcc aagaccgcgt atcgcgtcaa cctaaaactg gatcaggcgg
      661 acgtcgttga ttccggactt ccgaaagtgc gctacactca ggtatggtcg cacgacgtga
      721 caatcgttgc gaatagcacc gaggcctcgc gcaaatcgtt gtacgatttg accaagtccc
      781 tcgtcgcgac ctcgcaggtc gaagatcttg tcgtcaacct tgtgccgctg ggccgtgcgg
      841 atccgctagc ctccaaaacc atcgttcttt cggtcggcga ggctactcgc actctgactg
      901 agatccagtc caccgcagac cgtcagatct tc
//
