LOCUS       sequence-53863-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(N564K)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(316..339)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(363..387)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    428..446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    449..467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(469..672)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(498..518)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tttcgagacc tggtgcacag gaagaggccg aggtggccgg ggctttggtg tgggaggcga
       61 gatttttttc cttccaatat attctacata agttcccgga aagtcccccc tttcccctgt
      121 ggtttcatta tagccattta accagccaat ttcttcaggc ctggcttcct gtccatcact
      181 gaatccaaga gctactaagg accctttatt cacagtcaat atgtcaccca agtgcaagtc
      241 aatatcttct tctctttcct ttttataatc atacagcgct ctgtactggt acccctcagc
      301 actcatggcc atggatttgt catcatcgtc cttatagtcc atggtgaagg gggcggccgc
      361 ggagcctgct tttttgtaca aacttgttga tatctgcaga attccaccac actggactag
      421 tcgacgatct ctatcactga tagggagatc tctatcactg atagggagag ctctgcttat
      481 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      541 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      601 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      661 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      721 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      781 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      841 taaaatactc cacccattgg acg
//
