LOCUS       sequence-53864-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(R574fs)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(313..336)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(360..384)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    425..443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    446..464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(466..669)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(495..515)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gagacctggt gcaacaggaa gaggccgagg tggccggggc tttggtgtgg gaggcgagat
       61 ttttttcctt ccaatatatt ctacataagt tcccggaaag tccccccttt cccctgtggt
      121 ttcattatag ccatttaacc agccaatttc ttcaggcctg gcttcctgtc catcactgaa
      181 tccaagagct actaaggacc ctttattcac agtcaatatg tcacccaagt gcaagtcaat
      241 atcttcttct ctttcctttt tataatcata cagcgctctg tactggtacc cctcagcact
      301 catggccatg gatttgtcat catcgtcctt atagtccatg gtgaaggggg cggccgcgga
      361 gcctgctttt ttgtacaaac ttgttgatat ctgcagaatt ccaccacact ggactagtcg
      421 acgatctcta tcactgatag ggagatctct atcactgata gggagagctc tgcttatata
      481 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      541 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      601 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
      661 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
      721 ccataaggtc atgtactggg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      781 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      841 aatactccac ccattgacgt caaatggaaa agtcccctat ttggcggttt actatg
//
