LOCUS       sequence-53865-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(T576del)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(309..332)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(356..380)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    421..439
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    442..460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(462..665)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(491..511)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cctggtgcaa caggaagagg ccgaggtggc cggggctttg gtgtgggagg cgagattttt
       61 ttccttccaa tatattctac ataagttccc ggaaagtccc ccctttcccc tgtggtttca
      121 ttatagccat ttaaccagcc aatttcttca ggcctggctt cctgtccatc actgaatcca
      181 agagctacta aggacccttt attcacagtc aatatgtcac ccaagtgcaa gtcaatatct
      241 tcttctcttt cctttttata atcatacagc gctctgtact ggtacccctc agcactcatg
      301 gccatggatt tgtcatcatc gtccttatag tccatggtga agggggcggc cgcggagcct
      361 gcttttttgt acaaacttgt tgatatctgc agaattccac cacactggac tagtcgacga
      421 tctctatcac tgatagggag atctctatca ctgataggga gagctctgct tatatagacc
      481 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      541 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      601 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
      661 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
      721 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
      781 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
      841 tccacccatt gacgtcaatg gaaaagtccc tattggcgtt actatgggga acatacgtca
      901 ttatttgacg tcaatgggcg gggggtcgtt tgggcggtc
//
