LOCUS       sequence-53868-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(W583del)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(300..323)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(347..371)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    412..430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    433..451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(453..656)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(482..502)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaggaagag gccgaggtgg ccggggcttt ggtgtgggag gcgagatttt tttccttcca
       61 atatattcta cataagttcc cggaaagtcc cccctttccc ctgtggtttc attatagcca
      121 tttaaccagc caatttcttc aggcctggct tcctgtccat cactgaatcc aagagctact
      181 aaggaccctt tattcacagt caatatgtca cccaagtgca agtcaatatc ttcttctctt
      241 tcctttttat aatcatacag cgctctgtac tggtacccct cagcactcat ggccatggat
      301 ttgtcatcat cgtccttata gtccatggtg aagggggcgg ccgcggagcc tgcttttttg
      361 tacaaacttg ttgatatctg cagaattcca ccacactgga ctagtcgacg atctctatca
      421 ctgataggga gatctctatc actgataggg agagctctgc ttatatagac ctcccaccgt
      481 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      541 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      601 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      661 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
      721 tactgggcat aatgcccagg cgggccattt accgtcattg acgtcaatag gggggcgtac
      781 ttggcatatg ata
//
