LOCUS       sequence-53870-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST47-CypB-Del3 C-term GFP
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(617..641)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(673..692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(674..692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(737..836)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(766..786)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac tttgtacaag
       61 aaagctgggt tctactcctt ggcgatggca aggggcttct ccacctcgat cttgccgcag
      121 tctgcgatga tcacatcctt caggggttta tcccggctgt ctgtcttggt gctctccacc
      181 ttccgcacca cctccgtgcc ctctagaact ttgccaaaca ccacatgctt gccatctagc
      241 caggctgtct tgactgtcgt gatgaagaac tgggagccgt tggtgtcttt gcctgcgttg
      301 gccatgctca cccagccagg cccgtagtgc ttcagtttga agttctcatc ggggaagcgc
      361 tcaccgtaga tgctctttcc tcctgtgcca tctcccctgg tgaagtctcc gccctggatc
      421 atgaagtcct tgattacacg atggaatttg ctgtttttgt agccaaatcc tttctctcct
      481 gtagctaagg ccacaaaatt atccactgtt tttggaacag tctttccgaa gagactaaag
      541 atcacccggc ctacatcttc atctccaatt cgtaggtcaa aatacacctt gacggtgact
      601 ttgggcccca tggtgaagcc tgcttttttg tacaaacttg tttgatcaag cttaactagc
      661 cagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
      721 ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      781 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
      841 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatcccc
//
