LOCUS       sequence-53874-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault 
            M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, 
            Paques F, Rawlings DJ, Scharenberg AM
  TITLE     Coupling endonucleases with DNA end-processing enzymes to drive gene
            disruption.
  JOURNAL   Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 
            2012 Sep 2.
  PUBMED    22941364
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2177"; journalName: "Nat Methods"; date: "2012-10"; 
            volume: "9"; issue: "10"; pages: "973-5"
COMMENT     Sequence Label: pExodus
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..81
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(221..240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(222..240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(285..488)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(314..334)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        489..792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
       61 aactagaagg cacagtcgag gctgatcagc gggtttaaac gggccctcta gactcgagcg
      121 gccgccactg tgctggatat ctgcagaatt ccaccacact ggactagtgg atccgagctc
      181 ggtaccaagc ttaagtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta
      241 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
      301 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      361 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      421 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
      481 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
      541 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
      601 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
      661 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
      721 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
      781 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
      841 tattaataac tagt
//
