LOCUS       sequence-53876-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of codon-optimized Trex2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault 
            M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, 
            Paques F, Rawlings DJ, Scharenberg AM
  TITLE     Coupling endonucleases with DNA end-processing enzymes to drive gene
            disruption.
  JOURNAL   Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 
            2012 Sep 2.
  PUBMED    22941364
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2177"; journalName: "Nat Methods"; date: "2012-10"; 
            volume: "9"; issue: "10"; pages: "973-5"
COMMENT     Sequence Label: pExodus CMV.Trex2
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctagcatgt ctgagccacc tcgggctgag acctttgtat tcctggacct
      121 agaagccact gggctcccaa acatggaccc tgagattgca gagatatccc tttttgctgt
      181 tcaccgctct tccctggaga acccagaacg ggatgattct ggttccttgg tgctgccccg
      241 tgttctggac aagctcacac tgtgcatgtg cccggagcgc ccctttactg ccaaggccag
      301 tgagattact ggtttgagca gcgaaagcct gatgcactgc gggaaggctg gtttcaatgg
      361 cgctgtggta aggacactgc agggcttcct aagccgccag gagggcccca tctgccttgt
      421 ggcccacaat ggcttcgatt atgacttccc actgctgtgc acggagctac aacgtctggg
      481 tgcccatctg ccccaagaca ctgtctgcct ggacacactg cctgcattgc ggggcctgga
      541 ccgtgctcac agccacggca ccagggctca aggccgcaaa agctacagcc tggccagtct
      601 cttccaccgc tacttccagg ctgaacccag tgctgcccat tcagcagaag gtgatgtgca
      661 caccctgctt ctgatcttcc tgcatcgtgc tcctgagctg ctcgcctggg cagatgagca
      721 ggcccgcagc tgggctcata ttgagcccat gtacgtgcca cctgatggtc caagcctcga
      781 agcctgaatt ctgca
//
