LOCUS       sequence-53877-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of codon-optimized Artemis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault 
            M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, 
            Paques F, Rawlings DJ, Scharenberg AM
  TITLE     Coupling endonucleases with DNA end-processing enzymes to drive gene
            disruption.
  JOURNAL   Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 
            2012 Sep 2.
  PUBMED    22941364
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2177"; journalName: "Nat Methods"; date: "2012-10"; 
            volume: "9"; issue: "10"; pages: "973-5"
COMMENT     Sequence Label: pExodus CMV.Artemis
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcatgtc ctcatttgaa gggcagatgg cagaataccc caccattagc attgatagat
      121 ttgataggga aaacctcagg gcacgggctt atttcctgag ccactgccat aaggaccaca
      181 tgaaagggct cagggcacct accctcaaga ggagactgga gtgctccctc aaagtctacc
      241 tgtattgttc tccagtgaca aaggagctgc tcctgacttc ccccaaatat cgcttttgga
      301 agaaacgaat catttctatc gagattgaaa ctccaaccca gatcagtctg gtggatgagg
      361 cttcaggcga aaaggaggaa attgtggtca ccctcctgcc agcaggacac tgtccaggta
      421 gcgtcatgtt cctgtttcag ggcaacaatg gaaccgtgct gtacacaggc gacttccgcc
      481 tcgctcaggg agaggcagct cgaatggaac tcctgcattc tggcggacgg gtcaaggata
      541 tccagagtgt gtatctggac accacattct gcgatccccg gttttaccag attcctagcc
      601 gcgaggaatg tctgtccgga gtgctggagc tggtgaggtc atggatcacc agaagcccat
      661 atcacgtggt ctggctgaac tgcaaggcag cctacgggta tgagtacctc ttcacaaatc
      721 tgtccgagga actcggtgtg caggtccatg tgaacaaact ggacatgttt cgcaatatgc
      781 ccgagatcct ccaccatctg actaccgata gg
//
