LOCUS       sequence-53883-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: pLKO.1-sh-mSlug-4
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    134..251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        300..810
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     729..747
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(832..851)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gattctggct ttttatcttg tggaaggacg aaacaccggg cagacccact ctgatgtaaa
       61 ctcgagttta catcagagtg ggtctgcttt ttgaattctc gacctcgaga caaatggcag
      121 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      181 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      241 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
      301 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
      361 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
      421 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
      481 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
      541 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
      601 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
      661 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
      721 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
      781 gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac catgaccgag
      841 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
      901 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgnccacat
      961 cgagcggggt caccgaagct gcaagaactc ttc
//
