LOCUS       sequence-53893-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST47-CypB-Nucl2 C-term GFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(629..653)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(685..704)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(749..848)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(778..798)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagc tgggtgaaag ggcttctcca cctcgatctt gccgcagtct
      121 gcgatgatca catccttcag gggtttatcc cggctgtctg tcttggtgct ctccaccttc
      181 cgcaccacct ccatgccctc tagaactttg ccaaacacca catgcttgcc atctagccag
      241 gctgtcttga ctgtcgtgat gaagaactgg gagccgttgg tgtctttgcc tgcgttggcc
      301 atgctcaccc agccaggccc gtagtgcttc agtttgaagt tctcatcggg gaagcgctca
      361 ccgtagatgc tctttcctcc tgtgccatct cccctggtga agtctccgcc ctggatcatg
      421 aagtccttga ttacacgatg gaatttgctg tttttgtagc caaatccttt ctctcctgta
      481 gctaaggcca caaaattatc cactgttttt ggaacagtct ttccgaagag accaaagatc
      541 acccggccta catcttcatc tccaattcgt aggtcaaaat acaccttgac ggtgactttg
      601 ggccccttct tcttctcatc catggtgaag cctgcttttt tgtacaaact tgtttgatca
      661 agcttaacta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
      721 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
      781 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
      841 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
      901 gtgagtcaaa ccgctatcca cgcccattga tgtactg
//
