LOCUS       sequence-53897-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Wong KA, Lodish HF
  TITLE     A revised model for AMP-activated protein kinase structure: The 
            alpha-subunit binds to both the beta- and gamma-subunits although 
            there is no direct binding between the beta- and gamma-subunits.
  JOURNAL   J Biol Chem. 2006 Nov 24;281(47):36434-42. Epub 2006 Sep 29.
  PUBMED    17012231
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-24"; volume: "281(47)"; pages: "36434-42. 
            Epub 2006 Sep 29"
COMMENT     Sequence Label: AMPK-beta-2-FLAG
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..56)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      878..887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttcggtcct tattattagg taccgggccc tacttatcgt cgtcatcctt gtaatcctcg
       61 aggtcgacga tgggcttata cagcagcgtg gtgacatact tcttcttgta gcgatgggtt
      121 gcgctaagga ccatcacact gtccttaatg gacagtgcat agagatggtt cagcataaca
      181 tgattgggct caggaagtaa ggctgggtca catgaaatat tcgtgtcctt gttaagaata
      241 acttggagta ggtgaggggg caggatgggt ggggatttga atctctcctc agatcgaaac
      301 acatacattt cttgaccgta aggcccaggg ggtgagctgg acaggtctcg acatgacgtc
      361 tccgagcttt ccatagagtc taactttaaa gcgtcaaata cttcaaaatc agatttcttg
      421 acgtggatca agttattaat tgttccaagc tgactggtaa ccacaggctc tgacggatca
      481 tgaacccact gtccgtccac aaagaacttg tactgatgct ctccctctgg aagatccagg
      541 atggcaacga agtcattatg actctttatc agagggatct tggtgctcca attgttgaag
      601 gacccagaga tgaagacctc cttgcctcct tcagaccagc ggataacggt gggccgggcc
      661 tgctgggcgg gcttcacaga atcatccaaa tcctgctgcc agggtacaaa ctctttgtcc
      721 ccggggagct tggagtcggg caggctgaag acgctggggt cgtccgtgct ccccaccatg
      781 atcttgtgct cctttcccgg gccatggccg ccgccctcag ctcgagcagc tttggctccg
      841 tggcgctccc cggacacccg ctcgctggta gtgtttccca tggcggcggg ccaattcgtt
      901 tatcgaattc ctgcagcccg gggggatccg cccgggctaa agcggccgcc acgcgtgact
      961 cacttgtcct
//
