LOCUS       sequence-53898-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST47-CypB-Golgi C-term GFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(830..881)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(859..879)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac tttgtacaag
       61 aaagctgggt gaaagggctt ctccacctcg atcttgccgc agtctgcgat gatcacatcc
      121 ttcaggggtt tatcccggct gtctgtcttg gtgctctcca ccttccgcac cacctccatg
      181 ccctctagaa ctttgccaaa caccacatgc ttgccatcta gccaggctgt cttgactgtc
      241 gtgatgaaga actgggagcc gttggtgtct ttgcctgcgt tggccatgct cacccagcca
      301 ggcccgtagt gcttcagttt gaagttctca tcggggaagc gctcaccgta gatgctcttt
      361 cctcctgtgc catctcccct ggtgaagtct ccgccctgga tcatgaagtc cttgattaca
      421 cgatggaatt tgctgttttt gtagccaaat cctttctctc ctgtagctaa ggccacaaaa
      481 ttatccactg tttttggaac agtctttccg aagagaccaa agatcacccg gcctacatct
      541 tcatctccaa ttcgtaggtc aaaatacacc ttgacggtga ctttgggccc cttcttcttc
      601 tcatcggccg cagaaggtcc cggcagcagc aggaagaaga cggaccccgc gatgagggcg
      661 gcggcaagga gcaccttcat gttgcgttcg gagaggcgca gcatggtgaa gcctgctttt
      721 ttgtacaaac ttgtttgatc aagcttaact agccagcttg ggtctcccta tagtgagtcg
      781 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      841 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gaa
//
