LOCUS       sequence-53899-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Wong KA, Lodish HF
  TITLE     A revised model for AMP-activated protein kinase structure: The 
            alpha-subunit binds to both the beta- and gamma-subunits although 
            there is no direct binding between the beta- and gamma-subunits.
  JOURNAL   J Biol Chem. 2006 Nov 24;281(47):36434-42. Epub 2006 Sep 29.
  PUBMED    17012231
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-24"; volume: "281(47)"; pages: "36434-42. 
            Epub 2006 Sep 29"
COMMENT     Sequence Label: FLAG-AMPK-beta-2
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat gacgacgata
      121 aggcccgggc ggatcccccg ggctgcagga attcgccctt gatcatggga aacactacca
      181 gcgagcgggt gtccggggag cgccacggag ccaaagctgc tcgagctgag ggcggcggcc
      241 atggcccggg aaaggagcac aagatcatgg tggggagcac ggacgacccc agcgtcttca
      301 gcctgcccga ctccaagctc cccggggaca aagagtttgt accctggcag caggatttgg
      361 atgattctgt gaagcccgcc cagcaggccc ggcccaccgt tatccgctgg tctgaaggag
      421 gcaaggaggt cttcatctct gggtccttca acaattggag caccaagatc cctctgataa
      481 agagtcataa tgacttcgtt gccatcctgg atcttccaga gggagagcat cagtacaagt
      541 tctttgtgga cggacagtgg gttcatgatc cgtcagagcc tgtggttacc agtcagcttg
      601 gaacaattaa taacttgatc cacgtcaaga aatctgattt tgaagtattt gacgctttaa
      661 agttagactc tatggaaagc tcggagacgt catgtcgaga cctgtccagc tcaccccctg
      721 ggccttacgg tcaagaaatg tatgtgtttc gatctgagga gagattcaaa tccccaccca
      781 tcctgccccc tcacctactc caagttattc ttaacaagga cacgaatatt tcatgtgacc
      841 cagccttact tcctgagccc aatcatgtta tgctgaacca tctctatgca ctgtccatta
      901 aggacagtgt gatggtcc
//
