LOCUS       sequence-53905-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G
  TITLE     Microtubules induce self-organization of polarized PAR domains in 
            Caenorhabditis elegans zygotes.
  JOURNAL   Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354.
  PUBMED    21983565
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-10-9- 9"; volume: "13"; issue: "11"; 
            pages: "1361-7"
COMMENT     Sequence Label: pFM101
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..45)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(52..705)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(72..94)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(717..739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(752..758,759..774,775..779))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cttgctcacc atggatccca ggggcccctg gaacagaact tccagatccg attttggagg
       61 atggtcgcca ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta tatacttgct
      121 ggatttcaag tacttatcaa tttgtgggat agcttcaata cgttttttaa aacaaactaa
      181 ttttgggaac gcatccaggc acattgggtc catgtataaa acaacatcaa gagcgtcata
      241 caacatgaag tcaggatggg ttacatgatc accatttaaa tatgttttat gacataaacg
      301 atcttcgaac attttcagca tttcaggtag cttgctaaga aaatcaactt tgagagtttc
      361 aaagtcttta ctatatgcaa ttctcgaaac accgtatcta atatccaaaa ccgctccttc
      421 aagcattgaa atctctgcac gctcttttgg acaaccaccc aacatgttgt gcttgtcagc
      481 tatataacgt atgatggcca tagactgtgt taatttaaca tcaccatcaa tataataagg
      541 aagattggga aactccaaac ccaattcaaa ctttttgttt cgccatttat caccttcatc
      601 gcgctcatac aaatgctctt catatttttc ttcaagatat tccaaaagaa gtcgagtggg
      661 ttgcacaagg cccttaattt tccaataacc tagtataggg gacatgaata ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat taattgtca
//
