LOCUS       sequence-53911-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of enzyme-dead UTX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K
  TITLE     UTX regulates mesoderm differentiation of embryonic stem cells 
            independent of H3K27 demethylase activity.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4.
  PUBMED    22949634
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Sep 4."
COMMENT     Sequence Label: pCS2-UTX-F-MT2
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             56..85
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             95..124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             134..163
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             173..202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             212..241
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             275..304
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gatccgccgc catggctcca agaagaagcg taaggtaaat cgatcgaaag ctatggagca
       61 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga
      121 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
      181 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
      241 gaatgaaatg gagagcttgg gcgacctcac catggagcaa aagctcattt ctgaagagga
      301 cttgaattca aggcctctcg agatgaaatc ctgcggagtg tcgctcgcta ccgccgccgc
      361 tgccgccgcc gctttcggtg atgaggaaaa gaaaatggcg gcgggaaaag cgagcggcga
      421 gagcgaggag gcgtccccca gcctgacagc cgaggagagg gaggcgctcg gcggactgga
      481 cagccgcctc ttcgggttcg tgagatttca tgaagatggc gccaggacga aggccctact
      541 gggcaaggct gttcgctgct atgaatctct aatcttaaaa gctgaaggaa aagtggagtc
      601 tgatttcttt tgtcaattag gtcacttcaa cctcttattg gaagattatc caaaagcatt
      661 atctgcatac cagaggtact acagtttaca gtctgactac tggaagaatg ctgccttttt
      721 atatggtctt ggtttggtct acttccatta taatgcattt cagtgggcaa ttaaagcatt
      781 tcaggaggtg ctttatgttg atcccagctt ttgtcgagcc aaggaaattc atttacgact
      841 tgggcttatg ttcaaagtga acacagacta tgagtctagt ttaaagcatt ttcagttagc
      901 tttggttgac tgtaatccct gcactttgtc caatgctgaa attcaatttc acattgccca
      961 ct
//
