LOCUS       sequence-53918-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/Turkey_34_374
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..100
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             101..118
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
       61 aaatatagga tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatccgg
      121 tgacctgtta gttaaccctg aacagccgcg ccacaccatc ccaatcgctc agatcgctcc
      181 tgatctcata ctcgccgacc tgcctcgcaa tataatgctg aatactgatg agctggaatt
      241 tgataaagca ccagaatttc tgctgggcga cggcggtttc ggctctgtgt accgtgcttc
      301 ttatgctggc gaagaagtag ctgttaagat tttcaacaaa catactagcc tgcgtctgct
      361 gcgtcaagaa ctggccgtac tgtgccatct gcatcatcct tccctggtta gcctgctggc
      421 cgctgcagtc cgtccgcgca tgctggtcat ggagttagct cttaaaggct cactggatcg
      481 cctgctccag caggataaag ctaacctgac ccgcacgctc cagcaccgta tcgctctcca
      541 cgtagctgac ggcctgcgtt acctgcactc cgctatgatc atctaccgtg acttgaagcc
      601 gcataacgtc ctcctcttca ccctctaccc gaacgcagct gtgatagcta agattgccga
      661 ctacggcatc gctcaatact gctgccgcat gggcatcaag accagcgagg gcactccggg
      721 tttccgtgca ccggaagtag ctcgcggcaa cgtcatctac aaccaacagg ctgatgtgta
      781 cagctttggt ctgctcctgt atgacatact gactggtggc ggtcgcataa tggaaggtgt
      841 taaattccct aacgaatttg acgagctcgc tatc
//
