LOCUS       sequence-53920-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycDICER
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(77..94)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctatgacgtc gcatgcacgc gatcaaccac tttgtacaag aaagctgggt ctagatatct
       61 cgagtgcggc cgctcagtga tggtgatggt gatggctatt gggaacctga ggttgattag
      121 ctttgaggct tcggagggct cttcttgctg ctgcagattt ggcaatcctg taacttcgac
      181 caacaccttt aaatttcccc tttcctacta cttccacagt gactctgacc ttcccgtcat
      241 aagttctctc agccgggcta aatttggcag tttctggttc catttcaagc aattctcgca
      301 ctggggaacg gggtacattt gcagaaaact tttctattag tggccgcatc atgggatagt
      361 acacctgcca gactgtctcc agtgacatcc cactatccat gtaaatggca ccagcaagcg
      421 actcaaaaat atcccccatg gcctttggaa cttcaatatc ctcttctttc tcttcatcct
      481 cctcagatct cctaagctca gaatccattc cttgcatttc attcttctca agctgaaact
      541 gcacaaagtc atcaatgaca tggaagagct caggagagac agctttgaag tacttgtggt
      601 agtcgtactt tacagccagc gatgcaaaga tggtgttgtt gaccagggca gaccgcaggt
      661 ctgtcaggac ccccggggag tgctgccgcg ggtcttcata aaggtgcttg gttatgaggt
      721 agtccaaaat cgcatctccc aggaattcta agcgctggta acaatcagtg atagtattgt
      781 agtggtagga ggcatgtgta aaagcctgga gaaggtaagc cttattcttg aatctgtagt
      841 tgattttc
//
