LOCUS       sequence-53928-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pTvHR21-SGC
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        113..558
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat acatatggca ttcctgaaag
       61 atccataact tcgtatagca tacattatac gaagttatgc ggccgcgacg tccacatata
      121 cctgccgttc actattattt agtgaaatga gatattatga tattttctga attgtgatta
      181 aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa
      241 cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc
      301 aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg
      361 aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg
      421 gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta
      481 actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata
      541 aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc
      601 tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa
      661 aagccatata aggaaacata cggcatttcc catattacac gccatgatat gctgcaatcc
      721 ctgacagcaa aatgaaatat aagtctgatc
//
