LOCUS       sequence-53929-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/Dvl1_1_670
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..105
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             106..123
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
       61 cctataaata taggatctat gcaccaccat caccaccatc accacttggt gccccgcgga
      121 tccatggctg aaaccaagat tatctaccac atggacgagg aagagactcc atacctcgta
      181 aagctgccgg ttgctccaga acgtgtcact ctggctgact tcaagaacgt cctgagcaac
      241 cgcccggttc acgcatacaa attcttcttt aagtcaatgg accaggactt tggcgtagtt
      301 aaggaggaaa tcttcgacga taacgcaaag ctgccatgct ttaacggccg tgtagtatct
      361 tggctggttt tagctgaagg tgcacacagc gacgctggct cccaaggcac cgatagccac
      421 actgatctgc cgccacctct cgaacgcacc ggcggtatag gcgactctcg tccgccgagc
      481 ttccacccaa acgtagcttc ttcgcgtgac ggtatggata atgaaaccgg caccgaatct
      541 atggtctctc accgccgcga acgtgcccgt cgccgtaacc gtgaagaggc cgctcgcacc
      601 aacggtcacc cacgtggtga ccgtcgccgt gatgtaggcc tgccgcctga cagcgcttcc
      661 accgctctgt cctctgaact ggaaagctct agcttcgtgg attctgacga agacggtagc
      721 accagccgcc tgagcagctc caccgaacaa tccacttctt cccgtctgat ccgcaaacat
      781 aaacgtcgcc gtcgcaagca gcgcctccgc caggctgacc gtgcgtcctc tttctcctcc
      841 atcaccgact ccaccatgag cctgaacatc gttactgtga cctt
//
