LOCUS       sequence-53932-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/14-3-3_1_247
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..103
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             104..121
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
       61 tataaatata ggatctatgc accaccatca ccaccatcac cacttggtgc cccgcggatc
      121 catggttgat agggagcaat tagtccagaa agctcgctta gctgaacaag ctgaacgcta
      181 cgatgacatg gctgcagcta tgaagaacgt tacggagctg aatgaaccct taagtaacga
      241 agaacgtaac cttctgagcg ttgcatacaa gaacgtcgtt ggcgcccgtc gctcatcgtg
      301 gcgtgtgatc tcatccatag aacagaagac tagcgcagac ggtaacgaga aaaagatcga
      361 aatggttcgt gcttacaggg agaaaatcga gaaagagttg gaagctgtct gccaagacgt
      421 tctgagcctg cttgacaact acctgatcaa gaactgttcc gagacgcaat acgaatctaa
      481 agttttttac ctgaagatga aaggcgacta ctataggtat ctggctgagg ttgctaccgg
      541 tgagaaacgt gctaccgtag ttgaatcaag tgagaaggct tattcagaag cccacgaaat
      601 tagtaaggaa cacatgcaac ctacacaccc aatacgtctt ggcttagctc tgaactactc
      661 tgtattctac tacgagatac agaatgctcc tgaacaggct tgccaccttg ctaagactgc
      721 tttcgacgac gccatagctg agttagacac ccttaatgag gactcttaca aagatagcac
      781 actaattatg caactgctga gggataactt aaccctgtgg
//
