LOCUS       sequence-53934-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/14-3-3_1_247
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(784..801)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(802..825)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tggtggtggt ggtggtgctc gagtgcggcc gcaagcttat caattgttac cttcaccgcc
       61 gtcatcgtct tgttggtcgc tggtccacag ggttaagtta tccctcagca gttgcataat
      121 tagtgtgcta tctttgtaag agtcctcatt aagggtgtct aactcagcta tggcgtcgtc
      181 gaaagcagtc ttagcaaggt ggcaagcctg ttcaggagca ttctgtatct cgtagtagaa
      241 tacagagtag ttcagagcta agccaagacg tattgggtgt gtaggttgca tgtgttcctt
      301 actaatttcg tgggcttctg aataagcctt ctcacttgat tcaactacgg tagcacgttt
      361 ctcaccggta gcaacctcag ccagatacct atagtagtcg cctttcatct tcaggtaaaa
      421 aactttagat tcgtattgcg tctcggaaca gttcttgatc aggtagttgt caagcaggct
      481 cagaacgtct tggcagacag cttccaactc tttctcgatt ttctccctgt aagcacgaac
      541 catttcgatc tttttctcgt taccgtctgc gctagtcttc tgttctatgg atgagatcac
      601 acgccacgat gagcgacggg cgccaacgac gttcttgtat gcaacgctca gaaggttacg
      661 ttcttcgtta cttaagggtt cattcagctc cgtaacgttc ttcatagctg cagccatgtc
      721 atcgtagcgt tcagcttgtt cagctaagcg agctttctgg actaattgct ccctatcaac
      781 catggatccg cggggcacca agtggtgatg gtggtgatgg tggtgcatag atcctatatt
      841 tataggtttt
//
