LOCUS       sequence-53941-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             120..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             620..637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(690..708)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      704..751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggcgcggtga gccggcccga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
      121 ttgtttaact ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg
      181 tagatctggg taccgagaac ctgtacttcc aatccatggg caaactgagc gaacaactga
      241 agcattgcaa tggcattctt aaagagctcc tgagtaaaaa acacgccgcc tatgcgtggc
      301 cattttataa accggtggat gcctctgcgc tgggtctgca tgattatcac gatatcatta
      361 aacatccgat ggatctctca accgttaaac gtaaaatgga aaatcgcgat tatcgtgatg
      421 cccaggaatt tgcggcggat gtacgcctca tgttttcgaa ctgctacaaa tataaccctc
      481 cagatcacga tgttgtggca atggcacgaa agctacagga tgtatttgag ttccgttatg
      541 ccaagatgcc agattgacag taaaggtgga tacggatccg aattcgagct ccgtcgacaa
      601 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      661 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      721 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      781 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtgggtgg tttgcgcgca
      841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttt cttccttcct
      901 ttttctcggc
//
