LOCUS       sequence-53942-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1882_2527_M2397T
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccgag
      121 ttcctgctag gcgacggctc tttcggttca gtttaccgtg ctgcttacga gggtgaagag
      181 gttgccgtga agatttttaa caagcacact tcgctccgtc tgctgcgtca ggagcttgtg
      241 gttctctgcc acctgcacca cccttccctg atctccctgc tggccgccgg cattaggcct
      301 cgcatgctag tgatggaact ggccagtaag ggttcattgg accgcctgct gcagcaggac
      361 aaggctagcc tgacccgtac gctccagcac cgtatcgccc tgcatgtggc cgacggactg
      421 cgctacttgc acagtgccat gatcatttat cgcgatctga agcctcataa tgttcttctg
      481 ttcacgctct acccaaacgc agctatcatt gccaaaattg ccgattacgg catagcccag
      541 tactgctgtc gtatgggtat caagactagc gagggtacac ctgggttccg cgctcccgaa
      601 gtcgcacgcg ggaacgtgat ctacaaccag caggccgacg tgtactcgtt tggcctgctc
      661 ctgtatgaca ttctcactac cggaggccgg attgtggagg gcctgaaatt tcccaacgag
      721 ttcgacgagc tggagatcca gggcaagctg cctgaccccg tgaaggagta cggttgtgca
      781 ccctggccta tggtggagaa gctcattaag cagtgtctga aggagaaccc tcaggaacgc
      841 cccacaagcg cccaggtgtt cgacattc
//
