LOCUS       sequence-53944-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MINA
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccggcacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatggc agctgggggg ccttcagctt taaactttga
      241 cagtcccagt agtctctttg aaagtttaat ctcgcccatc aagacagaga cttttttcaa
      301 ggaattctgg gagcagaagc cccttctcat tcagagagat gaccctgcac tggccacata
      361 ctatgggtcc ctgttcaagc taacagatct gaagagtctg tgcagccggg ggatgtacta
      421 tggaagagat gtgaatgtct gccggtgtgt caatgggaag aagaaggttt taaataaaga
      481 tggcaaagca cactttcttc agctgagaaa agattttgat cagaaaaggg caacgattca
      541 gtttcaccaa cctcagagat ttaaggatga gctttggagg atccaggaga agctggaatg
      601 ttactttggc tccttggttg gctcgaatgt gtacataact cccgcaggat ctcagggcct
      661 gccgccccat tatgatgatg tcgaggtttt catcctgcag ctggagggag agaaacactg
      721 gcgcctctac caccccactg tgcccctggc acgagagtac agcgtggagg ccgaggaaag
      781 gatcggcagg ccggtgcatg agtttatgct gaagccgggt gatttgttgt actttcccag
      841 aggaaccatt catca
//
