LOCUS       sequence-53945-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1867_2527_M2397T
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..107
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gataaccatc tcgcaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
       61 aacctataaa tataggatct atgcaccacc atcaccacca tcaccacttg gtgccccgcg
      121 gatccaacat catgctgaac aatgacgagc tggagtttga acaagcaccc gagttcctgc
      181 taggcgacgg ctctttcggt tcagtttacc gtgctgctta cgagggtgaa gaggttgccg
      241 tgaagatttt taacaagcac acttcgctcc gtctgctgcg tcaggagctt gtggttctct
      301 gccacctgca ccacccttcc ctgatctccc tgctggccgc cggcattagg cctcgcatgc
      361 tagtgatgga actggccagt aagggttcat tggaccgcct gctgcagcag gacaaggcta
      421 gcctgacccg tacgctccag caccgtatcg ccctgcatgt ggccgacgga ctgcgctact
      481 tgcacagtgc catgatcatt tatcgcgatc tgaagcctca taatgttctt ctgttcacgc
      541 tctacccaaa cgcagctatc attgccaaaa ttgccgatta cggcatagcc cagtactgct
      601 gtcgtatggg tatcaagact agcgagggta cacctgggtt ccgcgctccc gaagtcgcac
      661 gcgggaacgt gatctacaac cagcaggccg acgtgtactc gtttggcctg ctcctgtatg
      721 acattctcac taccggaggc cggattgtgg agggcctgaa atttcccaac gagttcgacg
      781 agctggagat ccagggcaag ctgcctgacc ccgtgaagga gtacggttgt gcaccctggc
      841 ctatggtgga gaagctcatt aagcagtgtc
//
