LOCUS       sequence-53947-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA7
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gcacggctgg ggctacggcc aggacgacgg
      241 cccctcgcat tggcacaagc tgtatcccat tgcccaggga gatcgccaat cacccatcaa
      301 tatcatctcc agccaggctg tgtactctcc cagcctgcaa ccactggagc tttcctatga
      361 ggcctgcatg tccctcagca tcaccaacaa tggccactct gtccaggtag acttcaatga
      421 cagcgatgac cgaaccgtgg tgactggggg ccccctggaa gggccctacc gcctcaagca
      481 gtttcacttc cactggggca agaagcacga tgtgggttct gagcacacgg tggacggcaa
      541 gtccttcccc agcgagctgc atctggttca ctggaatgcc aagaagtaca gcacttttgg
      601 ggaggcggcc tcagcacctg atggcctggc tgtggttggt gtttttttgg agacaggaga
      661 cgagcacccc agcatgaatc gtctgacaga tgcgctctac atggtccggt tcaagggcac
      721 caaagcccag ttcagctgct tcaaccccaa gtgcctcctg cctgccagcc ggcactactg
      781 gacctacccg ggctctctga cgactccccc actcagtgag agtgtcacct ggattgtgct
      841 ccgggagccc atctgcatct ctgaaaggca gatggggaag ttccggagcc tgctttttac
      901 ctcggaggac gatgagagga tccacatggt gaca
//
