LOCUS       sequence-53948-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1847_2527_M2397T
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..91
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatatagg
       61 atctatgcac caccatcacc accatcacca cttggtgccc cgcggatcca ggctgaccat
      121 ccccatctcc cagattgcac ccgaccttat cctcgctgac ctgcctcgca acatcatgct
      181 gaacaatgac gagctggagt ttgaacaagc acccgagttc ctgctaggcg acggctcttt
      241 cggttcagtt taccgtgctg cttacgaggg tgaagaggtt gccgtgaaga tttttaacaa
      301 gcacacttcg ctccgtctgc tgcgtcagga gcttgtggtt ctctgccacc tgcaccaccc
      361 ttccctgatc tccctgctgg ccgccggcat taggcctcgc atgctagtga tggaactggc
      421 cagtaagggt tcattggacc gcctgctgca gcaggacaag gctagcctga cccgtacgct
      481 ccagcaccgt atcgccctgc atgtggccga cggactgcgc tacttgcaca gtgccatgat
      541 catttatcgc gatctgaagc ctcataatgt tcttctgttc acgctctacc caaacgcagc
      601 tatcattgcc aaaattgccg attacggcat agcccagtac tgctgtcgta tgggtatcaa
      661 gactagcgag ggtacacctg ggttccgcgc tcccgaagtc gcacgcggga acgtgatcta
      721 caaccagcag gccgacgtgt actcgtttgg cctgctcctg tatgacattc tcactaccg
//
