LOCUS       sequence-53953-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CRYBA4
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..208
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
      181 gggtaccgag aacctgtact tccaatccat gtcagcggga ccctggaaga tggtggtgtg
      241 ggatgaggac ggcttccagg gccggcggca cgagttcacg gccgagtgcc ccagcgtgct
      301 ggagcttggc ttcgagactg tgcgatcttt gaaagtgctg agtggagcgt gggtgggctt
      361 cgagcatgct ggcttccaag ggcagcagta cattctggaa cgaggcgaat atccaagctg
      421 ggatgcctgg ggcggcaaca cggcctaccc cgccgagagg ctcacctcct tccggcctgc
      481 ggcctgtgct aaccaccgtg actcgaggct gacaatcttc gagcaagaga acttcctggg
      541 caagaaagga gagctgagcg atgactatcc ttccctccag gccatgggat gggaaggcaa
      601 tgaagtaggg tccttccacg tccactctgg ggcctgggtt tgctcccagt ttccgggcta
      661 ccgaggattt cagtatgtgc tggaatgcga tcaccattcc ggtgactaca aacatt
//
